![]() ![]() However, abnormalities were noted in hamster spermatids and acrosomes in seminiferous tubules of recipient mice. In addition, cryopreserved hamster testis cells generated spermatogenesis in recipients. Approximately 6% of the tubules examined demonstrated xenogeneic spermatogenesis. In four separate experiments with a total of 13 recipient mice, hamster spermatogenesis was identified in the testes of each mouse. The hamster diverged at least 16 million years ago from the mouse and produces spermatozoa that are larger than, and have a shape distinctly different from, those of the mouse. In the present study, hamster donor testis cells were transplanted to mice to determine whether xenogeneic spermatogenesis would result. It was recently demonstrated that rat spermatogenesis can occur in the seminiferous tubules of an immunodeficient recipient mouse after transplantation of testis cells from a donor rat. Ogawa, T Dobrinski, I Avarbock, M R Brinster, R L Xenogeneic spermatogenesis following transplantation of hamster germ cells to mouse testes. STAT2 KO hamsters may be useful for addressing sexual transmission, pathogenesis, routes of fetal infection, and neurological disease outcomes, and may also be used in antiviral or vaccine studies to identify intervention strategies. Nevertheless, infectious virus and ZIKV RNA was detected in some, but not all, placentas and fetal brains of KO hamsters. ![]() Neonates exposed as fetuses to ZIKV at 8 days post-coitus were not smaller than controls. ZIKV-infected cells with morphologies of Sertoli cells and spermatogonia were observed in the testes, which may have implications for sexual transmission and male sterility. Subcutaneous injection of ZIKV of adults resulted in morbidity, mortality, and infection of the uterus, placenta, brain, spinal cord, and testicles, thus providing an opportunity to evaluate congenital ZIKV infection in a second rodent species besides mice. Zika virus (ZIKV) infection was investigated in adult and fetal STAT2 knock-out (KO) hamsters. Siddharthan, Venkatraman Van Wettere, Arnaud J Li, Rong Miao, Jinxin Wang, Zhongde Morrey, John D Julander, Justin G Zika virus infection of adult and fetal STAT2 knock-out hamsters. This humoral antibody is not detected during first pregnancy, and peritoneal exudate cells obtained from pregnant primiparous hamsters demonstrated a high degree of cytotoxicity. However, the cytotoxicity of peritoneal exudate cells from multiparous hamsters was greatly reduced during pregnancy, a time when noncytotoxic humoral antibody reactive with surface antigen of SV40 tumor cells is present. Peritoneal exudate cells from these donors reacted in similar fashion against SV40 tumor cells in vitro and in adoptive transfer tests in vivo. Lymph node cells from virgin female donors were inactive. Lymph node cells from nonpregnant primiparous and multiparous animals were cytotoxic in microcytotoxicity tests against SV40, polyoma, and adenovirus 7 tumor cells, but were not active against control BHK cells. The parity status of normal (uninoculated) hamsters also influenced the cytotoxicity of their lymphoid cells against tumor cells. Reppucci, Phyllis Dierlam, Peggy Rutala, William Coggin, Joseph H.įetal tissue from primiparous hamsters prevented simian virus 40 (SV40) tumorigenesis in male hamsters, whereas fetal tissue from multiparous hamsters did not. Prevention of Simian Virus 40 Tumors by Hamster Fetal Tissue: Influence of Parity Status of Donor Females on Immunogenicity of Fetal Tissue and on Immune Cell Cytotoxicity ![]()
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